See who you know at ion torrent by life technologies, leverage your professional network, and get hired. Roches 454 life sciences is partnering with dna electronics on the. Ion torrent have marketed their machine as a rapid, compact and economical sequencer that can be utilized in a large number of laboratories as a bench top machine. Scott parker scientist ion torrent by life technologies. Ion torrent has revolutionized dna sequencing by enabling a direct connection between chemical and digital information through the use of proven semiconductor technology. Life tech pushes speed of small, fast dna sequencer.
Despite several findings on hbc with ngs, a recent study demonstrated that with regard to the two most common platforms, neither the illumina miseq sequencer with the supplied miseq reporter software nor the life technologies ion torrent personal genome machine ion pgm with the supplied torrent suite software were completely suitable for. Thermo fisher has the resources to pour even more funding into the ion torrent line, but it is unknown what the new parent companys intentions are for life technologies, which comes with a huge portfolio of biotechnology products, any number of which will fit into thermo fishers existing pipelines in sample prep, synthetic biology, forensics. Being a part of life technologies, one might imagine, should give the chaps at ion torrent a decent amount of access to solid machines, and one more run of moores genome on a solid 4 would have. Here we compare the performance of these two technologies by investigating their ability to detect differentially expressed genes and pathways in a treatmentcontrol experimental paradigm involving the effect of il1. Life technologies ion torrent ion onetouch 2 biosurplus. Jul 05, 2012 the gc depth distribution is better in ion torrent from figure 1.
The company has developed a sequencing technology based on measuring the flow of hydrogen ions produced as new letters are added to a dna strand. Just before life technologies purchased the startup company ion torrent, the fledgling company was dealing with a torrent of another kindworldwide media interest in its new sequencing technology, which promised to bring the price of nextgeneration. Life technologies completes acquisition of ion torrent. General overview life technologies now thermofisher expanded their ngs portfolio with the acquisition of ion torrent and their first system, the ion personal genome machine pgm sequencer, in 2010. Page 2 the information in this guide is subject to change. How accurate is the new ion torrent genome, really. Ion torrents founder, jonathan rothberg, says that life technologies was particularly interested in his technology because of the potential diagnostic applications, though he is careful to note. However in hiseq 2000, the average sequencing depth is 4x when the gc content is 60%, while it is 3x with 70% gc content. Life technologies has four locations in california, including the headquarters in carlsbad, and three other locations in benicia, foster city and pleasanton 2.
Mar 22, 2016 thermo life technologies ion onetouch 2 w es torrent for sample preparation 9701 duration. Jun 26, 20 ion torrent continues to play a key role in our life technologies portfolio and is integral in the pursuit of our purpose of shaping discovery and improving life. The ion pgm sequencer must be connected to the ion pgm torrent server by a standard category 6 ethernet cable. Confidential for life technologies internal use only. Life technologies industryleading brands are found in nearly every life sciences lab in the world and include innovative instrument systems under the applied biosystems and ion torrent names, as. Page 2 the information in this guide is subject to change without notice. Lastly, life technologies manufacturers rna preparation kits and lifescope genomic analysis software optimized for use with ion torrent products and systems 6. A comparison of illumina and ion torrent sequencing platforms. Life moved into the nextgeneration sequencing market in 2010, by acquiring ion torrent and its semiconductor sequencing technology, which it has continued to develop.
Life technologies now thermofisher expanded their ngs portfolio with the acquisition of ion torrent and their first system, the ion personal genome machine pgm sequencer, in 2010. Life technologies 7469 ion torrent personal genome machine dx included components. Ngs systems are typically represented by solidion torrent pgm from life sciences. Life technologies debuts ion torrent machine, next. Thermo life technologies ion onetouch 2 with es torrent for. A treatmentcontrol experimental design to compare platforms. Dec 02, 2016 despite several findings on hbc with ngs, a recent study demonstrated that with regard to the two most common platforms, neither the illumina miseq sequencer with the supplied miseq reporter software nor the life technologies ion torrent personal genome machine ion pgm with the supplied torrent suite software were completely suitable for. While this technology uses a sequencingbysynthesis method and emulsion pcr empcr similar to other platforms, it differs in that it doesnt use fluorescence or chemiluminescence. Ion torrent has developed a dna sequencing system that directly translates chemical signals a, c, g, t into digital information 0, 1 on a semiconductor chip. Bgi partners with life technologies to offer faster, more. Thermo scientific ion torrent s5 user manual pdf download. Thermo life technologies ion onetouch 2 with es torrent.
Ion ampliseq technology is globally recognized as the goldstandard, ampliconbased enrichment method for targeted nextgeneration sequencing ngs. Dec 17, 2014 this video gives an overview on ion torrent sequencing, another type of next generational sequencing. Bgi partners with life technologies to offer faster, more affordable sequencing applications on ion torrent worlds largest genome center to purchase 50 ion proton sequencers from life. System includes 844126 ion one touch es dx ion torrent, ion one touch dx ion torrent, 6431 ion pgm dx chip minifuge, poweredge t430 computer, and accessories. View profile view profile badges get a job like scotts. Dec 08, 2010 ion torrents founder, jonathan rothberg, says that life technologies was particularly interested in his technology because of the potential diagnostic applications, though he is careful to note. Powered by semiconductor chips, ion torrent nextgeneration sequencing technology helps you implement a fast and simple workflow that scales to your research needs across multiple applications including inherited diseases, oncology, infectious diseases, reproductive genomics, human identification, and agrigenomics. Torrent suite software integration automate your data analysis needs and leverage the power of ion reporter software directly from within torrent suite software. Ion ampliseq ngs panels thermo fisher scientific us. Ion torrent pgm from life technologies choosing the ion torrent import will open the dialog shown in figure 6. We do not troubleshoot data transfer issues associated with an indirect connection between the ion pgm and the ion pgm torrent server. Importing data from ion torrent we support import of two kinds of data from the ion torrent system.
Life technologies 7469 ion torrent personal genome machine. The ion torrent pgm offers next generation sequencing at an affordable price. Using data from each instrument they created and compared the denovo assemblies of a known genome escherichia coli o104. In this video we are given a tour of the ion torrent laboratory at life technologies. Ion torrent, a whollyowned subsidiary of life technologies, has pioneered an entirely new approach to sequencing that enables a direct connection between chemical and digital information.
For the fastest, simplest, and most scalable ngs solution, there is no substitute. The technology was licensed from dna electronics ltd, developed by ion torrent systems inc. Life technologies ion torrent personal genome machine. Ion torrent personal genome by life technologies andbio. Page 1 user guide ion torrent s5 and ion torrent s5 sequencer catalog numbers a27211, a272 publication number man0010811 revision a. With fast development and wide applications of nextgeneration sequencing ngs technologies, genomic sequence information is within reach to aid the achievement of goals to decode life mysteries, make better crops, detect pathogens, and improve life qualities. Life technologies was acquired by thermo fisher scientific in january, 2014. Application guide ion ampliseq library preparation on the ngs express perkinelmer 6 required materials and reagents reagents reagent vendor and part number ion ampliseq library kit 2.
The ion torrent method uses the same idea as the small molecule real time sequencing where they sequence at enzymatic rate using the dna polymerase. In ion torrent, the sequencing depth is similar while the gc content is from 63% to 73%. Life technologies announces agreement to acquire ion torrent. The authors evaluate the sequencing data produced by three different instruments. Ion torrent continues to play a key role in our life technologies portfolio and is integral in the pursuit of our purpose of shaping discovery and improving life. Part of thermo fisher scientific, the world leader. Life technologies ion torrent personal genome machine sold asis. This video gives an overview on ion torrent sequencing, another type of next generational sequencing. Thermo fisher sheds businesses in preparation for life. Just select create an ion reporter workflow as you plan your sequencing runs and the software automates the rest from primary analysis, to data transfer, and secondary. Life technologies ion torrent ion onetouch es 844121 the ion onetouch es employs proven magnetic bead technology to isolate templatepositive ion sphere particles that can be loaded directly onto the ion semiconductor chipdelivering automated, highly reproducible enrichment with every run.
Ion torrent has already released ion 314 and 316 and planned to. We sought to compare the illumina and ion torrent sequencing platforms using a treatmentcontrol experimental paradigm see methods section for details. Ion torrent genexus system specimen to report in a single day with a handsoff, automated workflow the genexus system is the first turnkey ngs solution that automates the specimentoreport workflow and delivers results in a single day with just two user touchpoints. Life technologies corporation california in the nanoeconomy. Life technologiesion torrent ion onetouch es 844121 andbio. Feb 23, 2011 life tech pushes speed of small, fast dna sequencer.
However, it has struggled to gain market share from illumina, whose portfolio includes the cambridge university sequencing spinout solexa, which it acquired in 2006. Learn about working at ion torrent by life technologies. Thermo life technologies ion onetouch 2 w es torrent for sample preparation 9701 duration. We support import of two kinds of data from the ion torrent system. Jun 16, 20 in this video we are given a tour of the ion torrent laboratory at life technologies. Aug 10, 2017 both illumina and ion torrent provide alternative methods for researchers to study rna at the sequence level. The proteogenomics core now provides high throughput sequencing with the ion torrent personal genome machine pgm. Our innovative life science, applied science, and clinical products are now found under the applied biosystems, invitrogen, gibco, ion torrent, and molecular probes brands. Ion torrent made a splash with its launch at this years advances in genome biology and technology meeting in february heres my coverage from the meeting. In 2010, life technologies introduced the ion personal genome machine pgm as a postlight sequencing technology. Nov 02, 2012 lastly, life technologies manufacturers rna preparation kits and lifescope genomic analysis software optimized for use with ion torrent products and systems 6.
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